Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Chicken Lysozyme (also known as LZM/LYZ) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Chicken LZM/LYZ antibody. LZM/LYZ present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Chicken LZM/LYZ Antibody is added and binds to LZM/LYZ in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LZM/LYZ antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Chicken LZM/LYZ. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm